LOCUS       KO-first_condition_ready_26467_MGI:101781        21513 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Sntb1 targeting project(s): 26467
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 42225
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..8471
                     /note="5 arm"
     misc_feature    8499..8523
                     /note="B1 Gateway"
     misc_feature    8536..8583
                     /note="Frt"
     intron          8584..9458
                     /note="mouse En2 intron"
     misc_feature    9456..9458
                     /note="Splice Acceptor"
     exon            9459..9631
                     /note="mouse En2 exon"
     misc_feature    9643..10236
                     /note="ECMV IRES"
     gene            10237..13297
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13336..13579
                     /note="SV40 polyadenylation site"
     misc_feature    13583..13616
                     /note="loxP"
     promoter        13643..14162
                     /note="human beta actin promoter"
     gene            14188..14982
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15182..15423
                     /note="SV40 polyadenylation site"
     misc_feature    15440..15487
                     /note="Frt"
     misc_feature    15494..15527
                     /note="loxP"
     misc_feature    9473..9550
                     /note="FCHK std_gpr"
     misc_feature    15543..15563
                     /note="B2 Gateway"
     misc_feature    8472..15593
                     /note="Synthetic Cassette"
     exon            15989..16205
                     /db_xref="ENSEMBL:ENSMUSE00000473534"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000473534"
     misc_feature    15594..16375
                     /note="Critical Region"
     misc_feature    16399..16432
                     /note="Downstream LoxP"
     misc_feature    16376..16455
                     /note="synthetic loxP region"
     misc_feature    16456..21513
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacaatcc tttgtatgta aaaataaact gataaatgtt ggtgaatgag
     4321 caaaaattta gatcatcata gttacttatt actgtattct ctctgtcttt atattctact
     4381 ggctggttaa gagcatcact ctacattaaa tattattccc aagaaggcaa acgttcctca
     4441 aggccttgct gtgctatctt ttctatgtaa attactctaa ttatgtacca tataagaagt
     4501 gggtacttgg caagtatttg cttttaggct aagattttta tatgttatca gctctgcccc
     4561 acaagtggac taagccacat atttctagaa tttttcatgc ttaagcagga tatttagaat
     4621 attgcttgga ttatcaatga cggtaatcct ttgtgccttc aaaaatgtta ggcctatgtt
     4681 taatatattt ttctttaggc agttaagtct catttccagg aattctctct acaactggcc
     4741 agtgattgga ccactgtatt gctcactgat aatcattatt gctactatct aaaataaaat
     4801 ccaaacttct caacttttgt gcatctttca tctacgtggc atagccagat atcatgaaag
     4861 agcatctctg gataaacaaa gtaacgctct atcaattgct agccatgtga ccccctacat
     4921 gacaaacttc taagcatgaa tgattgatgt atatatgaac actgacctat gtgtttggta
     4981 gaagtaggaa aatcttacaa aggttttctg aaaatgataa ctatattttt atattttatt
     5041 gcctatgggc acatggactc aatgaaggaa tggtagccta gatatttttg tctacagacc
     5101 ctggattccc taacaaatat aaagtgagtc cttaaatagt aattgtgaag attaactata
     5161 ggacaatgtg taagtaacct ggttcaatat tctgctcatg attgagtgca ttacaaatgt
     5221 tttttcttat ccttcttgca aaccaacacc aaatttttct ctgctatgga attgtgatca
     5281 gagttacaaa tcagggtgtg ttatataaaa ggacagtcat gatccttctg tttcttaaca
     5341 tgatgttcat gttttaaggc tgagttcaaa tgccatttac ccacatagca ttcttgagtc
     5401 tccctctgaa gccatcactc tcatctccag ccagattgaa tgatttgaat gataagaatc
     5461 cattccaaaa acaaaaacaa aacaaaacaa aacaaaacaa aacaaaaacc tctatcccaa
     5521 ctgctaccag aatatcctat atcccagtaa gtgtaattcg acaacaaagc cacacaattt
     5581 tactgtctgc agaaataggg aaatgagtca cttttttatt ttttaaatta gcgtaattag
     5641 ggtattaaat accatataga caaagtctat agatttagta cattctttag ctccatcatc
     5701 ttcccacctt cttgtgacct ccagtcctcc cttgtgactc ctccatcttt ttttctcttc
     5761 tgtattccat gtgagccttc tcctagacac ccccatcctc catcagcact cttttctgac
     5821 attttcccat catcttcctg gaatttttaa aactttatcc acatttacac tcatacacat
     5881 gtacctgcac gcacacatac acactcacaa acacacacac acacacacac acaccacata
     5941 tacaaagccc acacgattta tggaagagta aaaatacaat gtttttaaag agtcccattc
     6001 tttccaactt tgtgtagcta aagtcagtct ctatggggct tctacacttg ttatgagcca
     6061 tgcctgtgct cctctttaaa cagattactc agtaataaca catttcttaa aatgatctgt
     6121 ttgtgagtca cctctcccct cctatgactt taatctctgc tgacaggttt gctgggagga
     6181 ctgagctgtg gattcctagt aaatactgga ttcgtgccta caggttcttt tctaagttct
     6241 cttactccta ttataaaact tggctctctc ttctccatgc catggcttct gtacttcttc
     6301 agtaggattt cctagaggca ctgatcctgt gggaggtcag gcagcaactt tgtagcctcc
     6361 caggctttgc cacctctatt acacagcttt gtggagcaag gaaatggtga cattctgtat
     6421 tcagccaatt cacagcagct atgatacagg ccaaggatcc agattcagta ccaaaagcat
     6481 ttaatagagt catcttcatg ttataacact aatagagtca tcttcatgtt ataacacttg
     6541 agtatggaaa ctatgcatta ctttctgtag cctccctaac ctggtttcag tcttacacca
     6601 tggaagcatc agcttctgaa atgacttatt caagccatcc cagagatttt gttcatttta
     6661 atccactgtc attggtaatg acctcagcta tgatctctgc ctgttatgca atagagaacc
     6721 agagtggaaa gctcatagag aggacataaa acctcgtatt gcatgttcct gaaatcttgg
     6781 tcgccctgcc aactcaaaaa atatctaagg aataaggcaa acatgagaaa aagcagaact
     6841 tctgtatgtt ctactcagac ttgttaccct ttataatctt taaaaagtaa tatatataaa
     6901 tgtagttaaa cataaagtac acaaaaaaga tagtaaatgg atagcaaaag cctatttcta
     6961 tcttgctggc ttttcattct attctctggt acaaactcct ttcaatattt tctagaattt
     7021 gtactaaaaa taactgaaga tatataaata tagatagtat atatatacac acacacatat
     7081 atatatgtat atgatgtata tgtatgtata tatacatgta gatatacata tatttataca
     7141 tacacacata tatatgtatg tatacttatg catatacata tagagagata aatgcataca
     7201 tgtctatata tatattcata tgcatatata agaagtatat aatcacaaat ttacatatac
     7261 ttctagggtt gttttagtgt tgatttttgg ttgttgagac agtgtttacc tgtgtgtagc
     7321 cctgattgtc ttggaacttg ctttatagac cagactggcc tcaaactcaa agatccacct
     7381 gcctctattt cccaagtgct gtgattaagg catatgcctc catacccatc ctatatttgt
     7441 gatttttata cacaaagata ttgcactgtc tgctttatgt tttccttaac catcaatctt
     7501 ccaagtcttg ctccatcaac atgtgcatat ctacctcatt gcaatgccta tagagattat
     7561 gatatattta acctctcaat tttctaactc ttagccttga gaaataatgg ctctactact
     7621 acagattttg caactagaat atagatgttt gtattcattt ctttgtatac atgatttcag
     7681 caccactgaa gttgaaaagt ctacccttgc ttctgcgcat aataacacga tcctgggcaa
     7741 tgatgtagct gctagaatct gtatgtgtgc attaaatcat agtctttcag cctgggttac
     7801 atttcacatc atctgcgcac ttgttttcag tcttccacaa tatgatttat aatcagaaaa
     7861 gatagacagt gtcacagatg gaaaaaaaag atacctgcaa tgctcatctg ctggacattg
     7921 taatagcaag ttctaagatt gacattgata tggaatttaa ataataggat aattgggttg
     7981 aaagaagcaa aaagcctgaa tcttgttcag aatgtaacag gctttgtgct aacagtatgc
     8041 ccagtgcaag tttctttcag aggcagagat ttttccaaaa atataacctt ctattctctt
     8101 gaaattttca tatgagtaca gtgtatttac attatctact tcccattctc ttccctttct
     8161 gactcttctg tgtcctttca actaccttct ttcaaattca tgaccgtgct caacttcatg
     8221 gcacatacat tcactcaaaa tctactgaat cccattagta ttgcttgtat gtacatgtgt
     8281 tcagggctga ccactaagat tgggaaaaca attgggggcc catctcaaga gagaactaat
     8341 tatccatcct tcagcaacca tgaccgccta tagctcttca tctaggagtt gagcccttgt
     8401 taaatattgc tccatccata ttggtatgtt aactggtgtg gtcttgtgaa gacacctata
     8461 ttgttgagag taaggcgcat aacgatacca cgatatcaac aagtttgtac aaaaaagcag
     8521 gctggcgccg gaaccgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     8581 ttcgaaccct ttcccacacc accctccaca cttgccccaa acactgccaa ctatgtagga
     8641 ggaaggggtt gggactaaca gaagaacccg ttgtggggaa gctgttggga gggtcacttt
     8701 atgttcttgc ccaaggtcag ttgggtggcc tgcttctgat gaggtggtcc caaggtctgg
     8761 ggtagaaggt gagagggaca ggccaccaag gtcagccccc cccccctatc ccataggagc
     8821 caggtccctc tcctggacag gaagactgaa ggggagatgc cagagactca gtgaagcctg
     8881 gggtacccta ttggagtcct tcaaggaaac aaacttggcc tcaccaggcc tcagccttgg
     8941 ctcctcctgg gaactctact gcccttggga tccccttgta gttgtgggtt acataggaag
     9001 ggggacggga ttccccttga ctggctagcc tactcttttc ttcagtcttc tccatctcct
     9061 ctcacctgtc tctcgaccct ttccctagga tagacttgga aaaagataag gggagaaaac
     9121 aaatgcaaac gaggccagaa agattttggc tgggcattcc ttccgctagc ttttattggg
     9181 atcccctagt ttgtgatagg ccttttagct acatctgcca atccatctca ttttcacaca
     9241 cacacacacc actttccttc tggtcagtgg gcacatgtcc agcctcaagt ttatatcacc
     9301 acccccaatg cccaacactt gtatggcctt gggcgggtca tccccccccc cacccccagt
     9361 atctgcaacc tcaagctagc ttgggtgcgt tggttgtgga taagtagcta gactccagca
     9421 accagtaacc tctgcccttt ctcctccatg acaaccaggt cccaggtccc gaaaaccaaa
     9481 gaagaagaac cctaacaaag aggacaagcg gcctcgcaca gccttcactg ctgagcagct
     9541 ccagaggctc aaggctgagt ttcagaccaa caggtacctg acagagcagc ggcgccagag
     9601 tctggcacag gagctcggta cccggaagat ctggactcta gagaattccg cccctctccc
     9661 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     9721 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     9781 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     9841 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     9901 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     9961 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
    10021 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
    10081 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
    10141 acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
    10201 tcctttgaaa aacacgatga taagcttgcc acaaccatgg aagatcccgt cgttttacaa
    10261 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    10321 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    10381 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
    10441 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
    10501 cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg
    10561 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
    10621 tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg
    10681 tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac
    10741 ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt
    10801 gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc
    10861 tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat
    10921 gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac
    10981 tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg
    11041 cctttcggcg gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt
    11101 ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg
    11161 gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc
    11221 cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga
    11281 ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg
    11341 gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat
    11401 ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa
    11461 gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg
    11521 ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt
    11581 gtgatcatct ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat
    11641 cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac
    11701 accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc
    11761 ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg
    11821 ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac
    11881 tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat
    11941 cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt
    12001 ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg
    12061 ccgcatccag cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc
    12121 gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg
    12181 cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc
    12241 gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg
    12301 caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg
    12361 cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc
    12421 gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt
    12481 aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat
    12541 aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac
    12601 attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg
    12661 gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat
    12721 gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc
    12781 cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg
    12841 gcgagcgata caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca
    12901 gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc
    12961 gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg
    13021 agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc
    13081 ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat
    13141 cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg
    13201 attggtggcg acgactcctg gagcccgtca gtatcggcgg aattccagct gagcgccggt
    13261 cgctaccatt accagttggt ctggtgtcaa aaataataat aaccgggcag gggggatcta
    13321 agctctagat aagtaatgat cataatcagc catatcacat ctgtagaggt tttacttgct
    13381 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
    13441 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    13501 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    13561 tcttatcatg tctggatccg gaataacttc gtatagcata cattatacga agttatgttt
    13621 aaacggcgcg ccccggaatt cgccttctgc aggagcgtac agaacccagg gccctggcac
    13681 ccgtgcagac cctggcccac cccacctggg cgctcagtgc ccaagagatg tccacaccta
    13741 ggatgtcccg cggtgggtgg ggggcccgag agacgggcag gccgggggca ggcctggcca
    13801 tgcggggccg aaccgggcac tgcccagcgt gggcgcgggg gccacggcgc gcgcccccag
    13861 cccccgggcc cagcacccca aggcggccaa cgccaaaact ctccctcctc ctcttcctca
    13921 atctcgctct cgctcttttt ttttttcgca aaaggagggg agagggggta aaaaaatgct
    13981 gcactgtgcg gcgaagccgg tgagtgagcg gcgcggggcc aatcagcgtg cgccgttccg
    14041 aaagttgcct tttatggctc gagcggccgc ggcggcgccc tataaaaccc agcggcgcga
    14101 cgcgccacca ccgccgagac cgcgtccgcc ccgcgagcac agagcctcgc ctttgccgat
    14161 cctctagagt cgagatccgc cgccaccatg attgaacaag atggattgca cgcaggttct
    14221 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
    14281 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
    14341 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
    14401 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
    14461 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
    14521 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
    14581 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
    14641 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
    14701 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
    14761 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
    14821 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
    14881 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
    14941 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
    15001 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
    15061 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
    15121 gcggggatct catgctggag ttcttcgccc accccccgga tctaagctct agataagtaa
    15181 tgatcataat cagccatatc acatctgtag aggttttact tgctttaaaa aacctcccac
    15241 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
    15301 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    15361 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    15421 tccgggggta ccgcgtcgag aagttcctat tccgaagttc ctattctcta gaaagtatag
    15481 gaacttcgtc gagataactt cgtatagcat acattatacg aagttatgtc gagatatcta
    15541 gacccagctt tcttgtacaa agtggttgat atctctatag tcgcagtagg cgggtgcctc
    15601 ttacaatatt acagaccttc tttgttgcat gagccttaaa tgtagtgttg taggtgttcc
    15661 agttggggat ggacactcac ctattctctg catttgggca agctgcagat ctctgtaact
    15721 gccttctgct gaaaagagaa ttttctttga tgaaggatga gaacggcact actctatggg
    15781 tactaagata aacattttat ctcattcttg cctgggttta cagaaccaga cttgtgttcc
    15841 ctcctattga gtgagccttg agtccaatta gacagctgtt gaccacaata tataagtgtc
    15901 atttatacat tgtatgctgt accacatatt ggtattccta aaaagtggta ttcctaaaag
    15961 tgtttcttgt cttctttttt ctttatagtg aagtatatga gagaagccac accttacgtg
    16021 aagaaagggt ccccggtgtc tgagattgga tgggaaacac ctccacctga gtctccaaga
    16081 ttagggggtg gctctgcaga gcctctgtca tcccagtcct tctccttcca tagagaccgg
    16141 aaaagcatcc ccctcaagat gtgttatgtc acccggaaca tgaccttggc tgaccctgaa
    16201 aacaggtaac gggggaaact ttcctagcct ctgaggttat gacagaattt gctgggttgt
    16261 gcttgtgtct gaaaagactc catggatctc atgaatagaa tgaaaggtcc tcccaccctc
    16321 cacgcctgag gaagatgcat attgaaagac agagattata ttccagccag tttccgagat
    16381 ggcgcaacgc aattaatgat aacttcgtat agcatacatt atacgaagtt atggtctgag
    16441 ctcgccatca gttcacgcaa ggtagacatt ccacctaatt caggacaatc tgcagtacag
    16501 taaaagcgaa gcagaagtta tggtctcatt tcattccaaa atgatttgat tgtaggaaaa
    16561 ggtaccaagg aagagaatta agaagagaag agggagaagg aaagaaaggc tgaggaacca
    16621 agccagcaaa tggggactct tggtgacctt tgcatccgca ttaaagtgct aaaaatagaa
    16681 gcagctctac tggtccttat ttaccacatg tgtgggacag ctgcaactcc cacgaatggg
    16741 aaggatttgg ctagggtcac agttgctgat gggaaaaagc caaagtgatt ggggcaggga
    16801 gaaaactgac tctttgcttc caaactccat gtgggtctta gttctcgtcc cagtgaatcc
    16861 acgctaagga tgccatttgg gcccttctgg ctccaaccac ctgtcacatc cctctgcact
    16921 cctccccagc acccatccta gtgacctcat accaatacaa ggatgggctg cttagaagag
    16981 agagccagca gcagcagcag cagtgagaga ggaatcaagc caggtggaat gatgaggtag
    17041 tcctcccacg atgatctccc tcctccctgt tctgctttgt ccctcctctt tttccactga
    17101 ttccctgggc ctgcagttta tttcttgact gctcagaatt ttctcctccc tggggtcctc
    17161 ccctcttaat caaataaccc ctttctctca agaatctaat gtcaagtgtc agatctaata
    17221 aattcacaat ctgcagtatg ttcctcttgt actaggttgt atgttaactc atttagtcgg
    17281 gcaattgcaa agtcatagct tacagaggca ctcaactcat acatgttcct tttcatagtc
    17341 tgtgtaatag tctaactacc atttaaataa atctgaatta gttgtcgtat tgaatattgt
    17401 gacatttcac atttaaaaaa taatgttttt catttcttta aaaaaaaaca aaaacaaaaa
    17461 caaaaacaaa aacaaaaaca aaaaacaagt agctactaca ggcaggggtt taagtaacag
    17521 gagcccagga gtggtaaatc ctttgacaag gggatgaacg tactttctca ttcatgttgt
    17581 tcataattag agacttcaca gaattccatg cctggtcctc atattacctg cttaatacct
    17641 gacttcttca tactcggtag tcctcctaac agttgacgtg ttattgttgc actaattcta
    17701 caagttaaaa aaaaaaaaaa aaaaaaaaaa aggccgggca gtggtggcgc acgcctttaa
    17761 tcccagcact caggaggcag aggcaggcag atttctgagt tcaaggcttc aaggccagcc
    17821 agaactaaac agagaaaccc tatcttgaaa agaaaaagaa aagaaagaaa aaaaaacaaa
    17881 aaaaaaaaca aaaaaaggaa gatagagaaa tttccagaaa catcctagaa gttattaata
    17941 tcttggaaaa caaaagttca tgaatgtttt tttttctgct gttctttcta acaaaagact
    18001 tatatgttat aacatttatg acctataaac aacacggggt caatcaatta tcagagtgtt
    18061 ttgagaaacc tctggcttta ataatactgg gatttccatt atgtgtatct acatagacag
    18121 tgggatggat ttttaaagct taatgccact gctgtctaag tctaccgctg atgagttatc
    18181 tacagagcgt cctgggtggg agttgcagag cctgttctgc taggatatga gaaacaacag
    18241 ggcagaatta aaagcaaaga tccagctgag acagatgggt tctgaggaga catcaagtcc
    18301 agataggtcc cgaactactg ctaccaagta gatgagggta gaacttgccc ccagtaaagg
    18361 aaaaaaaaaa aaaaagaaag aaaaccaaaa taggaaacaa atactttata gaattgcatt
    18421 tgcctgctgg gaaaaatata gcattgccaa tcagacagat aatgtagcca aatcacagtt
    18481 ctgcctagta caagaaacac tccaaacaca aactgatttc agctgtgaaa gacacagcac
    18541 ctgtcaggag cccccaaaac caatgcaaag gagcagcaac tgaaagcacc aatttctttt
    18601 tttttaattt ttttattggt tattttattt attaacattt caaatactat ccttcttacc
    18661 ggtttcccct ctgaaaaccc cctatcgcac ccctcctcct ccctgattct atgagggtgc
    18721 tcccctaccc acccattcac tcccatatca ctgccctagc agtcccctat gtggggtcat
    18781 gaacccttca caggaccaag cccccaccac caccattgat gccaaataag gctatcctct
    18841 gctacatgca gctggagcca tcctccatgt atattctttg gttggtggtt tagtccctgg
    18901 gagctctggg aaggttctgg ttggttgcta ttgttcctcc tatggggctg caaacccctt
    18961 caggtccttc agttcttccc ctaactcctc cattgcaatt tcaatcttga tatatgacaa
    19021 ggtaaagatg caggccacag aacacaaaac agaaccaaga agggctgatt tatgctgaag
    19081 accaaaattc acaagaagta ttagatagat ttaaacacaa accaaataat aattcagcag
    19141 taacttgtat agcagaagct atagaaagtt gaggaaacag aagcatggta caggaattta
    19201 atccaagtca ctctcagtcc aagatgggtc acttggaagg caaaagatga ataaggatat
    19261 agatttaaat agtataagaa gtggctccca gatgcataat aaaattcgta cctggctatt
    19321 ggaaaatgaa ctttatactt aaaggcaaag tttacaacca taacacctag acccttccaa
    19381 tctcatttga tctcaaatac acacagaaca tgcatacagt taggcaaaga gtgtacatca
    19441 aagagaatgt aataggtccc atacactaga aatagtagag acaatgtcct ctggcagtag
    19501 caggttaaaa ccagaaaatt aataataaaa tcaaacctaa aatacctttc tttttgagtt
    19561 ttgtaaaaca ccctctataa gcaatcttgg gaccactgaa gaaatacaca gtaaacttct
    19621 agcccatctt taaatgataa caatgtaaac tggcacacag tgttaatata aaaaagagga
    19681 aagcacccac cctcacctac tgctgcacaa ggtgagggag ggggtggaac acctagcccg
    19741 ggcagcacag tagggctggc cctggtgtct gggatgggta agccagccct gagattctga
    19801 gcaggagagt tagtcctgat agtgtggatg caggagagca ggtgggctga ccaacttagc
    19861 ttgcactcag gcccagatcc aggcctctga gttggtccag cccatcatct accccaacta
    19921 gaacttgtaa gagggctggt ctcagtccca caggtcctga aaatctgaag ctacgggatc
    19981 tccataacat agggcaacaa caggatatct gagaggtatc acttatgatt gggtattgat
    20041 ggtgtagcag aagccagagg ccttgaacca gaccaatgac tcatcacaat gatcattcag
    20101 gactaaagct gttcgggcaa agggatgtag tgtgggacac attgagacac tcactgcagg
    20161 ttccacacta agaaggatgt ttgtttgttt gtttgtttgt ttgtttgtgt ttaggggcgg
    20221 ttatttttaa tttttgtttt gttttgggaa gaaaggttgc aaggacagtg gttgaataca
    20281 aagagatgaa gagatgatcg ggattggagt gcatgatgtg acattcataa aaaagggaaa
    20341 aaaggaaagg agagaaaggg atcagaaagt gtgtactgtg tgtgtctagc cacagtcata
    20401 gtgaaatgaa gtcttagttc tgccacatct tcatcaattt aagcaagaac tcatcgtggg
    20461 atgcaattaa ccatcttcta catagtctat ctccacattg caagctttgc tgaggattgg
    20521 gtgtaaaaac aaacagaagc cttggctgat accaaaccag tttctgaatc attacctcct
    20581 ttgtctccaa cctcaacacc tggaggaaca gttcactggg atactgtcag ccgggagtta
    20641 atgggtccac gggtgccaga acatgaaaac ggagtgacaa atgccacagc atcttagggg
    20701 cttgatgccc tcagtggggg gacacacagt tgctcacagt ttacattaaa acctttaatg
    20761 ctcaatttct caaacagaaa cagccacaga acagcaggca acatcgctgt tctttctgtg
    20821 gaaaaggtca ctcagcaggt accaaatcca caagcatttc actaatagtg gccaccggtg
    20881 aggacatcct tcttccaaat gctacctctt cacatatccc ctacttttca tccttaattc
    20941 ttggcctcca cacccattgt cctcatcctg ggccacatct tccgcccacc agagtgtctg
    21001 gagccatgtc ctttctggtg cttttttttt tttcagattg taacgtctcc agtctctcat
    21061 cctcctagtc aatggaaaaa gaaacccagc gttttaccat ttgctcaaat aggcccccac
    21121 tcctctgcat aataatgtct atcctcttta gtattgaggg ggaaagcccc cagtaagctg
    21181 tgtgtctgtt ttaattgctc agcactggtc cctaatttct agcctttatg cttcagctat
    21241 ccttaggcag tttgcatagc ccagcagtca cctgcctgtc atcttcccaa aggcccttat
    21301 ttaagcccag cagaagcaac ccctctggga catggctttc ttgtgataca ctttccttgc
    21361 cactctggct gttattgcaa ccttcttaaa gactgacaac tttttctgtg cacctaccta
    21421 gttcccaagc tatatagttc ttgaaatcgg atatgttcca cgttttcatt ccattacccc
    21481 agctcttagg aacattgttt agtacaaagt agc
//