LOCUS       KO-first_condition_ready_27233_MGI:101780        22291 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Serpine2 targeting project(s): 27233
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_st1
COMMENT     design_id: 47174
COMMENT     backbone: L3L4_pZero_DTA_kan
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     promoter        85..598
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             612..1268
                     /note="DTA"
     misc_feature    1393..1651
                     /note="BGH pA"
     CDS             complement(2147..2941)
                     /note="KanR"
     misc_feature    4053..4073
                     /note="B3 Gateway"
     misc_feature    4079..4086
                     /note="AsiSI"
     misc_feature    1..4111
                     /note="Synthetic Backbone"
     misc_feature    4112..9482
                     /note="5 arm"
     misc_feature    9510..9534
                     /note="B1 Gateway"
     misc_feature    9547..9594
                     /note="Frt"
     intron          9595..10469
                     /note="mouse En2 intron"
     misc_feature    10467..10469
                     /note="Splice Acceptor"
     exon            10470..10516
                     /note="mouse En2 exon"
     misc_feature    10517..11194
                     /note="rat Cd4"
     misc_feature    10999..11082
                     /note="rat CD4 transmembrane region"
     gene            11203..14249
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14263..14316
                     /note="'self-cleaving' 2A peptide"
     gene            14317..15108
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15308..15549
                     /note="SV40 polyadenylation site"
     misc_feature    15566..15613
                     /note="Frt"
     misc_feature    15620..15653
                     /note="loxP"
     misc_feature    10484..10534
                     /note="FCHK std_st2"
     misc_feature    10484..10501
                     /note="FCHK QC prefix region"
     misc_feature    10502..10516
                     /note="FCHK QC critical region"
     misc_feature    10517..10534
                     /note="FCHK QC suffix region"
     misc_feature    15669..15689
                     /note="B2 Gateway"
     misc_feature    9483..15719
                     /note="Synthetic Cassette"
     exon            16223..16421
                     /db_xref="ENSEMBL:ENSMUSE00000157299"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 1 ENSMUSE00000157299"
     misc_feature    15720..16765
                     /note="Critical Region"
     misc_feature    16789..16822
                     /note="Downstream LoxP"
     misc_feature    16766..16845
                     /note="synthetic loxP region"
     exon            19736..19822
                     /db_xref="ENSEMBL:ENSMUSE00000157307"
                     /rank="7"
                     /note="ENSMUSE00000157307"
     exon            17734..17834
                     /db_xref="ENSEMBL:ENSMUSE00000157300"
                     /rank="6"
                     /note="ENSMUSE00000157300"
     misc_feature    16846..22291
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagataaacg
       61 gatcccctag tttgtgatag gatcgaattc taccgggtag gggaggcgct tttcccaagg
      121 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
      181 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
      241 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
      301 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
      361 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
      421 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
      481 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
      541 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      601 aggtcctcgc catggatcct gatgatgttg ttgattcttc taaatctttt gtgatggaaa
      661 acttttcttc gtaccacggg actaaacctg gttatgtaga ttccattcaa aaaggtatac
      721 aaaagccaaa atctggtaca caaggaaatt atgacgatga ttggaaaggg ttttatagta
      781 ccgacaataa atacgacgct gcgggatact ctgtagataa tgaaaacccg ctctctggaa
      841 aagctggagg cgtggtcaaa gtgacgtatc caggactgac gaaggttctc gcactaaaag
      901 tggataatgc cgaaactatt aagaaagagt taggtttaag tctcactgaa ccgttgatgg
      961 agcaagtcgg aacggaagag tttatcaaaa ggttcggtga tggtgcttcg cgtgtagtgc
     1021 tcagccttcc cttcgctgag gggagttcta gcgttgaata tattaataac tgggaacagg
     1081 cgaaagcgtt aagcgtagaa cttgagatta attttgaaac ccgtggaaaa cgtggccaag
     1141 atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa tcgtgtcagg cgatctcttt
     1201 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1261 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1321 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgca
     1381 gatcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacgcg ccgtacccag
     1681 cttttgttcc ctttagtgag ggcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     1741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2101 ctattggtta aaaaatgagc tgatttaaca aaaattttaa caaaattcag aagaactcgt
     2161 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     2221 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     2281 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2341 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     2401 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     2461 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     2521 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     2581 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     2641 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     2701 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     2761 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     2821 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     2881 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     2941 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     3001 ttggcggcga gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     3061 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     3121 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     3181 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     3241 tgaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3961 ttttacggtt cctgggcttt tgctggcctt ttgctcacat gtaatacgac tcactatagg
     4021 gcgaattgga gctaggcggc cgtttatcgt gccaacttta ttatacaaag ttgcggacgc
     4081 gatcgccggc ggataacaat ttcacacagg atagcacaga taaccacgac ttttacattt
     4141 cctctttcct gtctatatgt cagctttcaa ttctccagtc tatcctggcc cctgggtccc
     4201 aaatagtccc tttgattttt ctgtatcttt cttaaaaaaa aaaaaaagat ttgtctttta
     4261 attctgtgta tgcctatata tagatgccca cagtggccaa aagagggtat ctgataccct
     4321 ggagctctgg agtatctgaa ttctcatcct ctacaagggc agagcactgg gatgaaccac
     4381 tgggccatct ctccagcccc taaatatgtc cctttgcata ttcaatcacc cttacatcca
     4441 ggtgaccatg gcgctggctc cctgtggtag aagttaagtc atggctcctg agtgtattat
     4501 cagaacactt tctatcttgt cagtcagcag tcttctggga gcacactttc caaatgtgct
     4561 attcgataag tgttcattgg aaaatatcgt gtacttaaac gccacaggaa gtttatccca
     4621 aatacctgag cataggaagt agctcctttt gcctgaaagt cactgcagtc acactaagcc
     4681 ctccctgctg ggctgggctg ctggagaaca ccttgggatt ctgacagcat cctagtacat
     4741 agccaggcag aaatgctata ttttaaataa aatctttata cagtgggcct gcaaatcaaa
     4801 acaggagatt aaaatatgat gattctttag atattcctct gggagattca gacatgtaaa
     4861 tttttatagg aatatagtat gctcttcttg gctatataat ctattctcaa agagaaacac
     4921 ttaagggcat atacaaagtt cagaaccaat ttgtccaaga acatacaggt gttcacagat
     4981 gcgaatgatt gaacccagga ccctgggcag gctagacaaa gactaatggc atccttgata
     5041 taaatctatt ttatcagttc agatagagag gggagtgaga aaggagacac acagacaaac
     5101 agggagggag ggagggaggg agggagggag ggagggaggg agggagggag ggagggaggg
     5161 agggagggag ggaggatggg agggaggatg ggagagagga gaatgtgaga atgagtgtct
     5221 ctatctccct tcccttattc cctctccttt cctgcctcct tcacctcagg gtctgcagct
     5281 gagtcttcct gatggtgctc cccttttccc tggcttccta tgggagtggc cagtatagaa
     5341 acagcaggtg actaatgaga gaggagagaa ggctgtggct ttaacttccc agtctctccc
     5401 tcaatggcac caaggctgca ggcttcttac ccttcagggt ctcagctcct tccggagcct
     5461 gagcatgcga tcaggggctc tttccctcta ggtcacccac tccacaaggc tgggagtggt
     5521 gaccaccatt tataacctat ctgtagcagt ttccccatgt tcttcctgtg ccttatagaa
     5581 tgccttcatt aagatatgct tagatgaccc agcctggcat gattgcttca ggcccactgt
     5641 gactgctgta gagagctcag gatagactat gtgagtgttc ttcttgcctc tgtcctgttt
     5701 cattacaagt cagtacagtg ggcagcctgg gctcatcctt cttgccctcc tatctgtgtc
     5761 tggcattggg tctagctgga attccagctt ggagtctggc ttccttctgg gttgtgtgat
     5821 gtgagactgt catacatccc ctagattcac aggttgccac cacagcagct tatcaggata
     5881 agatcggctt atatgcatag gcctgtgagg agggtccagc aggcttcagt ggctcaacca
     5941 ggcattcatt tttatgactg cagacccctt agcctgttgg tcctttccgc ccatatatac
     6001 ttatcttctg ccaacactac cagccgggac tcaagaggct gctgtgggca gaacccacat
     6061 gactcacagg gccttctgtt gggtgttttc tgggactctg ccaagtcttg gcccccttgc
     6121 tgaaacactg ccgttcggag aggatctgac ttttcttctc ttccaagatg tcattggtgt
     6181 ctctgaagag acccgcctgg caacatggaa gaaaattaag agggagagaa atacttctga
     6241 cttatgcctc ttaagagaga ccttcaagaa gagacccttt ttaattatta ttatttattt
     6301 agaaagagct ttgaagtgct atttagtgaa agagcatttc aggctcgcgt gctgcaaagc
     6361 cagctgccac atgtataaag cgggaagaaa atggttccaa tagcctcact tcctagggaa
     6421 aaggcctcta ttaagtgcta ggcacatagt tttttatctt ttctatgaat atgtgaaata
     6481 tattttgaag cctttttgca tgcacaggat tacgctgcag tgtttattcc gtaatctatc
     6541 tggttcatta gctactgtat catggaatat tttttcagga atttgttagg atttacaaga
     6601 ctgattttta gctgcacgtt atttcatggt ataaacttac ataatatttt ctggctagtc
     6661 agttgcttct ttcctctaat aaataatcac cgagtttttc ttttgcatac atttgtgaag
     6721 gcttttatca tttattccta caggaaattt gcttaaatgg gaattgctga tcaaagttat
     6781 taaaaatgtt cagcataaat gcttgaattt atactataaa gatattatcc caggggaggg
     6841 agacaacaaa taatcatatt gctctctgtc tctctctctg tgtgtgtgtg tgtgtgtgtg
     6901 tgtgtgtgtg tgtgtgtgtg tgtgtgagtg gtcactgagt ttccaggacc cacctctttc
     6961 tgcttcccta gaactggaat tgcaagcttg gtaccctatc tggacttttc tatgggtact
     7021 gggaattgaa ctcagaccat catacttctg tcgaaggcac tctaccaact ggggtgtctc
     7081 cactgtccac ctatctcagg tttccttctt tctctgtttt cagctcgtat tcatccacct
     7141 attgtacgtc ccctttggct gtctactaag ttgtagttcc caagactagt tgtattctgt
     7201 tcttgcctgt aagatccccc aatagagcac ttctattggc tcacccagtt cttgggcctt
     7261 gtttcttccc cataagctgt ctccaggcct gctttggact ccagtctgtg atgtagacaa
     7321 ccatactcaa gaggatctgt ttgtcgtttc caaacacatt tttcactttc tattcaagtg
     7381 ggatgaggaa accctccaaa taccctttcc tgtgtgactt atcctaacag ggagatgtca
     7441 caccctctgc tgcaggtgtg gtttcccatg gtaagtcagc tgtgtctaca ctctgaggag
     7501 taaccaacca gtccttcaag acgtaaggca cctaagcacc tcatgtcctg tgctttgtca
     7561 tccccacatc tagctgaggc agaaggatct cgtgcttggg gtcagcctgg aatctccagc
     7621 aagactgtgt ttcagaaatg ggtcagtgac cacaaagctg ctcccatcag tatgaaagtc
     7681 ggtgctatgt gccgacttgg gctgagtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     7741 cgcacgcgta tgcatgtgtg tactgtcatg ctgagtttta ctaatgtcat tgactgaggg
     7801 agctatacag attggctttg aatctgggtt cttcagttta aacctgtgct ctcaatctca
     7861 tattggccct ctgtaaaagg ctatgggggg ggggggggca ggggaagctg tcctgtagtc
     7921 cctgggatgg aagagagagc acaggtttcc atccatctcc cgacttctca agatgacaag
     7981 ttcagcatgg atatggacag atgtagccta cctgctgaca gtggtcaccc agtcctgggg
     8041 tccagtgcag aggtagaaaa aggtcaaacg tccagtgaat gtgtgagtac acagaatagt
     8101 taactgtgct ggttctactg gtcagtaatg gtgtatccag tcaaggccgg gtgtgagcct
     8161 gcttaccgcc ccagctgcca cccactccag ctcatactca tactcgcatc actcacagct
     8221 tccatcctcc ttagctgaat tatacacact tctgcagcaa ttgctctctc caggtcttcc
     8281 ttttctgccc cagagcaata atttaataac tacttcctgc atttcacagt ttctaaaaat
     8341 tagccccatt agagccagag acttgatgtc ttctttgagg attaaagggc tcattaggaa
     8401 gtggggggta ttttcccact ttctcattga gttttcagtt tctactgaga ccgttttatc
     8461 actcaggcta aaagtaaaga cgaagtatat tcattttgct tcttgggtag tcccggcctg
     8521 gaatcgagac tggtgtgagc tcacagcaat tcagagctga gaccgatggc actgggttcc
     8581 ttggctgtcc tacaacccag tgcaatctct gcttggggag aagagatccc agctgtccct
     8641 ggaatatgat ggcagcagct cttctagcgc cttcagctga tggtgtttta tagctaaaag
     8701 tgcttgtgac agaacagggc ttgctaactt tctacctctt gctgtcttct ttctttcttt
     8761 gatctccttt cttcttctct ggaggtgtgg cctgttcaaa attctccagc ttgtagtggt
     8821 gtacactctt aatcccagaa ctcaggaagc agaggcgggg agatctctgt gcattcgagg
     8881 ctaacctggt ctacagagcg agttccagga tagccagggc tgttatacag tgaaacattg
     8941 cctcaaaaac aaacaaacaa ggccagccac caagaaaaaa cgagtgaaaa tactacgaac
     9001 atgttgcctc ttttctcttt gttgtacttc atatagggga agaaaggggg ggccccatgg
     9061 gcaccctggt tactactgca tctataattt tttttcattt gatcatctca gcaacctcgt
     9121 ggtccatact ttaatattcc gatatttcca tctgtataga cggatggcat ggggagaaca
     9181 tggtacctcc tcaagctcat ggagttcatc tttagaggtt cagtgcttaa cacacacttg
     9241 agaaattgtc ccatcggttt agaatgccaa caaggacttt ttgactgctc tgcggtcata
     9301 ctgcacagtt gctgtctctg gccgttctct gttgctgtgt gagttccttt tcttgttgct
     9361 ataagaaaat tcctgacaaa ggcagctaaa ggcaggaagg gtttcgtttt ggcttgtggt
     9421 ttgaggatat agtctattta tacctataat ttgtggtggc agtggtttga gttagctggt
     9481 caaaggcgca taacgatacc acgatatcaa caagtttgta caaaaaagca ggctggcgcc
     9541 ggaaccgaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcgaaccc
     9601 tttcccacac caccctccac acttgcccca aacactgcca actatgtagg aggaaggggt
     9661 tgggactaac agaagaaccc gttgtgggga agctgttggg agggtcactt tatgttcttg
     9721 cccaaggtca gttgggtggc ctgcttctga tgaggtggtc ccaaggtctg gggtagaagg
     9781 tgagagggac aggccaccaa ggtcagcccc ccccccctat cccataggag ccaggtccct
     9841 ctcctggaca ggaagactga aggggagatg ccagagactc agtgaagcct ggggtaccct
     9901 attggagtcc ttcaaggaaa caaacttggc ctcaccaggc ctcagccttg gctcctcctg
     9961 ggaactctac tgcccttggg atccccttgt agttgtgggt tacataggaa gggggacggg
    10021 attccccttg actggctagc ctactctttt cttcagtctt ctccatctcc tctcacctgt
    10081 ctctcgaccc tttccctagg atagacttgg aaaaagataa ggggagaaaa caaatgcaaa
    10141 cgaggccaga aagattttgg ctgggcattc cttccgctag cttttattgg gatcccctag
    10201 tttgtgatag gccttttagc tacatctgcc aatccatctc attttcacac acacacacac
    10261 cactttcctt ctggtcagtg ggcacatgtc cagcctcaag tttatatcac cacccccaat
    10321 gcccaacact tgtatggcct tgggcgggtc atcccccccc ccacccccag tatctgcaac
    10381 ctcaagctag cttgggtgcg ttggttgtgg ataagtagct agactccagc aaccagtaac
    10441 ctctgccctt tctcctccat gacaaccagg tcccaggtcc cgaaaaccaa agaagaagaa
    10501 cgcagatcgc agatctgcgg gctgcaggga gagttgagat ggaaggcaga gaaggctcct
    10561 tcttcccagt cctggatcac cttctcccta aagaaccaaa aggtgtctgt gcagaagtct
    10621 actagcaacc ccaagttcca gctgtccgaa acgctcccac tcacccttca gataccccag
    10681 gtctcccttc agtttgctgg ttctggcaac ctgaccctga ctctggacag agggatactg
    10741 tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc ccgacagcaa cactttgacc
    10801 tgtgaggtga tgggacccac ctcacccaag atgagactga tcttgaagca ggagaatcag
    10861 gaggccaggg tctccaggca ggagaaagtg attcaagtgc aggcccctga agcaggggtg
    10921 tggcaatgtc tactgagtga aggtgaagag gtcaagatgg actccaagat ccaggtttta
    10981 tccaaagggt tgaaccagac aatgttcctg gctgtcgtgc tggggagcgc cttcagcttt
    11041 ctggttttca cggggctctg catcctattc tgtgtcaggt gccggcacca acagcgccag
    11101 gcagcacgga tgtctcagat caagaggctt ctcagtgaga agaagacttg ccagtgctcc
    11161 caccggatgc agaaaagcca caatctcata tatgggagcg atgatcccgt cgttttacaa
    11221 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    11281 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    11341 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
    11401 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
    11461 cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg
    11521 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
    11581 tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg
    11641 tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac
    11701 ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt
    11761 gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc
    11821 tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat
    11881 gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac
    11941 tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg
    12001 cctttcggcg gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt
    12061 ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg
    12121 gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc
    12181 cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga
    12241 ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg
    12301 gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat
    12361 ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa
    12421 gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg
    12481 ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt
    12541 gtgatcatct ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat
    12601 cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac
    12661 accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc
    12721 ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg
    12781 ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac
    12841 tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat
    12901 cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt
    12961 ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg
    13021 ccgcatccag cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc
    13081 gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg
    13141 cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc
    13201 gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg
    13261 caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg
    13321 cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc
    13381 gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt
    13441 aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat
    13501 aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac
    13561 attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg
    13621 gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat
    13681 gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc
    13741 cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg
    13801 gcgagcgata caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca
    13861 gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc
    13921 gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg
    13981 agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc
    14041 ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat
    14101 cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg
    14161 attggtggcg acgactcctg gagcccgtca gtatcggcgg aattccagct gagcgccggt
    14221 cgctaccatt accagttggt ctggtgtcag gggatccccc gggagggcag aggaagtctt
    14281 ctaacatgcg gtgacgtgga ggagaatccc ggccctattg aacaagatgg attgcacgca
    14341 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
    14401 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
    14461 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
    14521 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
    14581 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
    14641 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
    14701 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
    14761 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
    14821 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
    14881 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
    14941 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
    15001 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
    15061 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
    15121 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
    15181 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
    15241 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc cccggatcta agctctagat
    15301 aagtaatgat cataatcagc catatcacat ctgtagaggt tttacttgct ttaaaaaacc
    15361 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    15421 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    15481 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    15541 tctggatccg ggggtaccgc gtcgagaagt tcctattccg aagttcctat tctctagaaa
    15601 gtataggaac ttcgtcgaga taacttcgta tagcatacat tatacgaagt tatgtcgaga
    15661 tatctagacc cagctttctt gtacaaagtg gttgatatct ctatagtcgc agtaggcggg
    15721 aaatgctttt accgtgtcct gccctaactg tcatattgta taccttaaat acaataccat
    15781 gtattttcta aaacaaagta gaatgtaaaa aaaaaaacaa aacaaaacaa aacaaaaaaa
    15841 aaaaacaaaa aaacaaagca agctcaaaag tagctttaat ttattctttt tctcgtataa
    15901 aggttaactt cttacgaaga actgtgcggc gagtaccgaa cagtctaatc gtctacttac
    15961 attcaactca gcagaatgaa agtgagctta gcggtgccca gaggcattct gtctagactg
    16021 aagacagaga attctgcacc agggaagaaa gaagtcattg tccttatctt tgtccttctg
    16081 agggagctat ttgtagcaaa gggagtttta caggctgaat tactgtctct ctcctacggc
    16141 ttccttggtg accgccagcc atcccccctc ccccccacag aagtcattag gcataaacta
    16201 gatctgcctc tggtttcttt agggtctacc aggaccccga atggcttatg gtacaacttc
    16261 attgagctgc cctaccatgg tgagagcatc agcatgctga tcgccctgcc aacagagagc
    16321 tccaccccac tgtctgccat catccctcac atcactacca agaccattga tagctggatg
    16381 aacaccatgg tacccaagag gatgcagctg gtcctaccca agtgagtgcc ccttccctta
    16441 aaaagaaagg atgttcttct caggggatag ctgtcatatt gtatactaga aacggggtgc
    16501 taagatggtc ttatggaaaa tgtgctttag agagaaccag gctgtctttt ctgaatgctt
    16561 tctctgagac cctgcagtgc ctgtttgtgg tgagacaaca aggatactaa aggagagcgg
    16621 gcagccctgg ccttagaacc cccatgaggg caaagtcttc atgactctgt gacaactttt
    16681 gacatatctc tttccattgg agaattgaaa aatacataca tatcttcata tggaatggat
    16741 tagaacacag tagtcgaagg agcttgagat ggcgcaacgc aattaatgat aacttcgtat
    16801 agcatacatt atacgaagtt atggtctgag ctcgccatca gttcaaggcc catgagactg
    16861 tattcatcag tatttaaggc agctttcctt ttatacccct caagtaaatt atagatggaa
    16921 tttctgatgt tatgactgga gactagggca aaagcaaacc caattctgtg tacatcatca
    16981 tttctaacag ttcaaagaac accttctctc tccagtgtgt gtgtgtgtgt gtgtgtgtgt
    17041 ggagggggag aaatctgtct ccttcctgtg aatttttcat cattaccagc tttcccacaa
    17101 tccagagttt ctatccatac ctcagtggaa acagagaatc ctaataactt ttaagtcttg
    17161 aatagtcaat gtaataatct acaaatgtag atttgtagca atctacaaat atcagaagtc
    17221 ctcctcctga gggggtgaag aagtgactcc atggttaaga gtcctcactg atctcgcaag
    17281 ggacctggat tctgccttaa ctcctacgtg gtgtcacaca accatctata actctagttc
    17341 caggggatct gatgcccttt cctggcttcc ttggggccag atgtataggc acatacatgc
    17401 atataggaaa aatacttata catttaaaag ataaaagtaa agacatctta aatgttaaat
    17461 gtactcttct agaaagccac tcaataatgt ttagctaaat atctgatatg gtcttcttgt
    17521 cacacctgga tggccatgct cattgtgagt aagaagaggg tgagttgtct acttacctgg
    17581 catttaactg ttaggtttag aagtcaggct gcagttcaga gtgacgacaa ggtcttactt
    17641 aaagagcctg tttcaaacta tgccctgatt ttgtcatgtt cgaatcattt atagcatgtt
    17701 ttaatcctcc cctatatttt ctctttcctg taggttcaca gctgtggcac aaacagatct
    17761 gaaggagcca ctgaaagccc ttggcattac tgagatgttt gagccatcaa aggcaaattt
    17821 tacaaaaata acaagtatgt caagtttaaa atgtaaattt tgagaaaatg ttacaggtcc
    17881 ttctttgctc aaaaaaagaa ataggaaaat cgggagtatg ttgccctaaa cctgtgctaa
    17941 ttttagtttg atgcattgcc aaaggatgcc gttgagtgcc tacggctcag ttagcattcg
    18001 tcctttcctc ctgcatctca tctgaggcct gaaggaccac caggataagg acttctttct
    18061 gcattgcctg tttacccaaa cacttggctt tttttccttc cctgatgctg ggacttgaac
    18121 ccacgtctgt gtgcttgcta gacaagtgct ctactgctga ggacctgtgt aacccttgag
    18181 tttgcctctt aattaggact gagttgtttt ttgttttttg tttttttaat ataaattttc
    18241 cccttgggtt tgggaaaaaa aaaaaaaaaa ctttaagatt ttcgaattct caaacattta
    18301 gcttacttcc acagagcaac tactgtaggc ttgccttgta tccagaaatg tattttgcca
    18361 tttgtctgaa gtaacgaatt caatacctct cattgagact gtccttcacc caagcagcta
    18421 ttcataacac atacctagca tcctctttgt gtatttgcca ttaggcctag aattgtttga
    18481 atgggtcagg tctctgacgt ctacatttag aacacaattt ccaatgacga gctatagctt
    18541 ggacatgcaa agtactctgg tgtgtatttt aacccttgac tggtagagag gtagttttat
    18601 taccaacccc agttgcgtgc tcaagagtat ctgaatatct cctgcgggag aagcgcctgc
    18661 cccctcaccc ctaacccacc ctcacacctt cgctggaact gaagctccac tgatccatct
    18721 tcccattctc tttcattcct ggtccccagc ccagcacatt agcataacct gttcaaaatc
    18781 ctcttaggaa tgcgtttctt ggttcggatc cagatccctg aatcacacct ttattttaat
    18841 gggatccttg gcctaataaa ctttgagaaa gaagcgcttc ttgttcaaaa tctcctgtta
    18901 tagtctgggt actaagtagg aggcccaggc accctgaatc ttcttgagct gctctcttta
    18961 acctggtagg tgacctgagc cacataaaca gtcacctggt ctcagctagc catggtagag
    19021 ctggctattt gtgcagagag agagagagag aagttctttt gttcttttta ccagacccat
    19081 atcccatcat gctcatgctg acaagagttg gtgtcctttt gggtagtagc agccctggga
    19141 attgtgcggt tgtttttgga cctgagggaa ctgaatccac tggaggatcc ccaaggcaca
    19201 gctgcagttc tgtgcctggc ccttcccagg agttccctcc tgatattgct gtgctggcag
    19261 atagtgtggc tatgaaagcc agcacttctg gagcctaact aaccgtggga ctagggacag
    19321 atttcttaac cttgtgagcc tcagtttccc catctgaaac cctctctggg atagacagtc
    19381 gagttagatt atattattgt ctttctactt ctgggggaaa aaatggcatc tttatagatc
    19441 tttgttcttt tcatttttaa aataaaatta atagtcatct gagttgaagt gattagaggc
    19501 actaaaacat ttagggtctg ggaatccatc ccagaagtaa caattgcaaa tcatttagca
    19561 gaaattgaaa tctactgcaa gtgacagcca cgcacggccc tcagtctgca ggactatcga
    19621 gttacttctg acccttttca taagctggaa tttgccgtgg ctttgaggtt actcctgata
    19681 actctagatg gcatgctcat caaagaagtg ctgattttgt gttttctggg aacagggtca
    19741 gagagccttc atgtctctca catcttgcaa aaagcaaaaa ttgaagtcag tgaagatgga
    19801 accaaagctt cggcagcaac aagtaagtgc tggctcacgt ggcaaggcca cgccaatagg
    19861 aaagcagaca cactccaggt tcactctgca gctcagctga cttctaggga gtcttgaaag
    19921 ctgacccttg ggagacagca atgagtatga actaatgaag tagttcatca taatgaggag
    19981 gaggaggagg aagaggagga ggaaggagga ggagaaggat gttgaggaag agataggaag
    20041 gcgccttgga gacaccaatg tgtatgggta aagtgtttga tgatggtggt gatgatgacg
    20101 acaatgttga tatcaggggt gtagctcagt ggtgtgagta cttgcccagc atacacaagg
    20161 ctatgggttt gaacacttaa cactgacaaa aaataaatca tgttagtgta ggaatttcat
    20221 ctaggaaagg ggtaccattt ccctggaaag agctagctag catgttattt cagccatgca
    20281 gccctcactg tcttcagccc aaaacctcaa gtctgcaaat acagcaagaa agcaaccaga
    20341 ggcatgcaac tgagtagata tggctcgccc agtcttctaa taaaacttta tttatcaaac
    20401 caggggttgg cagtgtagac ctagccctag taccattgca tgttggtccc tgctctcagc
    20461 aggaaaaagc atgtgtggct actttaccaa ccagaaaggc tcatctttag attgaaaggc
    20521 aacagcaagt acaggagcat agctgctcca gtctccccag gacaatgtga agaatcagca
    20581 tcgtctacag gacagcacca ggattgctgg gcagccttct ttgatcatta gaacatctgc
    20641 atctctcccc ttggcagatt ataaggaagg aagacctaag catgtctcca ccagcaccag
    20701 caataccatc aatcgtccat gttaaaaaga ttggtttatg agattgtaaa ctcataaact
    20761 agtttcactg aacatcctgc tgagcgattg actatttcta gtcatgaaac aaagttatca
    20821 tttattctac agagttagca ccttatcacc tccacaataa atgcttattt tttttcctgt
    20881 tgctttaaaa tagcacttaa cataaggacc tggaatacta gttttgattt tttttttaat
    20941 accctttgtc ttctctaggt gacggcttca tgttaaaatg atttgtccgt cttgccaact
    21001 accattccag tcaggacatg atttatagag gagctctgtg tgaatggaaa tgtagactcc
    21061 cttgttctaa attttactag ggatctcaag accactacag cagaatgtta aaccaaatcc
    21121 ggggctctgt gtcagcaggc ttcggccgct aaattagtca gctcagggtc ccctaatgat
    21181 gtcagaaacc agagaactta aacaacagat gtctattttt cccacagctc ttgaggccag
    21241 aagtctgggg ttggagtggc agcagggttg gcttctcttg aggtctttgt gtgctcatga
    21301 gacttgtgtg ctcatgtggt tttgtcatgt ccctggagat gctccccatc gtaataagga
    21361 tatcaggcct gttgggttgg tgcctcacct ttaggtcctt ctgtatcctt agtagactct
    21421 gtgaagggcc tgtctccatg tgtagtcata taaggagcct agcgcctcaa tggaggagtg
    21481 tcaccagtct acactgcatg ctctctaact gtaattctgg gtaaagcagt ctgcccagca
    21541 taacttccca gtattccttt tctcagagca agccacatca cttatgcttt tcaaaatact
    21601 gagccagtgt gccagacact agtagttata ctacccttct ctctttcctt agccttacca
    21661 tggtctttat taaacagggc caaccattgg tcaaagtagg cagatctaag acttacatgt
    21721 ctaaagaatc tttctttcag gcgagggtgt cgttcggtag caaataactt gcttagtggt
    21781 cacattgtcc tgggttttat ccccactata ttacaagaat aacaaaaagt tgttttctaa
    21841 gtcagatatc tgcagccaat gagttgagcg agtggtggtg taatatagca ggtgcagatg
    21901 agcagtctgt gttgctgtca caaaggacat ctaaatgatg taccaaagga gaagacgcag
    21961 agccgacagt taagcattct gagctggcct ttgatatgca gaaaggatgt gggtaggttg
    22021 aagaggtcca gaggctttat cagcacagca gatggtgtga gtaaaaatag cttataataa
    22081 cagcaaatat tctctgggtg ctcatttggt gccagtctct gtcccaagag ctgtagtgtg
    22141 tttgtcattc actccccata aatctatgaa agctaagaac atttattgtg aagatggggc
    22201 ttttatcgaa tatgtgagac agtaactgaa aaggccatcc caataaatag tgaccattga
    22261 gaccattata acatgccagc ttagaagaca a
//