Mesenteric Lymph Node Immunophenotyping: Images associated with FACS analysis

WT Images

16 weeks old

Naive CD8+ T cells - % of CD8+: 83.98

16 weeks old

Germinal centre B cells - % of CD45+: 0.564

16 weeks old

CD103+ CD11b-low cDC - % of CD45+: 0.367

16 weeks old

Conventional DC (cDC) - % of DC: 84.824

16 weeks old

CD11b-low cDC - % of cDC: 78.143

16 weeks old

Effector NK cells - % of CD45+: 0.188

16 weeks old

Total ab T cells - % of total T cells: 97.723

16 weeks old

Eosinophils - % of CD45+: 0.082

16 weeks old

Resting CD8+ T cells - % of CD45+: 3.191

16 weeks old

Total gd T cells - % of total T cells: 2.277

16 weeks old

Late germinal centre B cells - % of CD45+: 0.215

16 weeks old

Total NKT cells - % of CD45+: 0.358

16 weeks old

Effector CD4+ NKT cells - % of CD45+: 0.108

16 weeks old

CD4+ T helper cells - % of CD4+: 86.767

16 weeks old

Germinal centre B cells - % of B2 cells: 1.446

16 weeks old

Plasmacytoid DC - % of DC: 15.176

16 weeks old

CD11b-high cDC - % of cDC: 21.518

16 weeks old

Total gd T cells - % of CD45+: 1.301

16 weeks old

Total B2 cells - % of CD45+: 39.036

16 weeks old

Conventional DC (cDC) - % of CD45+: 0.692

16 weeks old

Early germinal centre B cells - % of CD45+: 0.35

16 weeks old

Total CD4+ T cells - % of CD45+: 31.099

16 weeks old

KLRG1+ CD4- NKT cells - % of CD45+: 0.002

16 weeks old

Granulocytes - % of CD45+: 0.015

16 weeks old

KLRG1+ Treg cells - % of CD45+: 0.114

16 weeks old

Resting NK cells - % of NK cells: 60.353

16 weeks old

KLRG1+ CD4+ T helper cells - % of CD45+: 0.073

16 weeks old

Total ab T cells - % of CD45+: 55.836

16 weeks old

Total NK cells - % of CD45+: 0.472

16 weeks old

Resting CD8+ T cells - % of CD8+: 13.092

16 weeks old

Resting gd T cells - % of CD45+: 1.083

16 weeks old

Naive CD8+ T cells - % of CD45+: 20.47

16 weeks old

KLRG1+ CD8+ T cells - % of CD8+: 0.161

16 weeks old

Resting NK cells - % of CD45+: 0.285

16 weeks old

Total DC - % of CD45+: 0.816

16 weeks old

CD5+ gd T cells - % of CD45+: 0.832

16 weeks old

Follicular B cells - % of CD45+: 37.52

16 weeks old

CD11b-low cDC - % of CD45+: 0.541

16 weeks old

KLRG1+ Treg cells - % of Treg: 2.76

16 weeks old

Plasma cells - % of B2 cells: 0.061

16 weeks old

CD4+ T helper cells - % of CD45+: 26.983

16 weeks old

KLRG1+ NK cells - % of NK cells: 13.428

16 weeks old

Total B cells - % of CD45+: 39.969

16 weeks old

KLRG1+ CD4+ T helper cells - % of CD4+ T helper cells: 0.269

16 weeks old

Resting Treg cells - % of Treg: 40.51

16 weeks old

Plasmacytoid DC - % of CD45+: 0.124

16 weeks old

Effector CD4+ T helper cells - % of CD45+: 4.258

16 weeks old

Effector gd T cells - % of gd T cells: 13.682

16 weeks old

Monocytes - % of CD45+: 0.09

16 weeks old

KLRG1+ CD8+ T cells - % of CD45+: 0.039

Mutant Images

16 weeks old

Nipsnap3atm1b(KOMP)Wtsi homozygote

Effector CD4+ T helper cells - % of CD45+: 5.348

16 weeks old

Nipsnap3atm1b(KOMP)Wtsi homozygote

Effector CD4+ T helper cells - % of CD45+: 4.011

15 weeks old

Nipsnap3atm1b(KOMP)Wtsi homozygote

Effector CD4+ T helper cells - % of CD45+: 3.16

15 weeks old

Nipsnap3atm1b(KOMP)Wtsi homozygote

Effector CD4+ T helper cells - % of CD45+: 5.386

15 weeks old

Nipsnap3atm1b(KOMP)Wtsi homozygote

Effector CD4+ T helper cells - % of CD45+: 3.145

15 weeks old

Nipsnap3atm1b(KOMP)Wtsi homozygote

Effector CD4+ T helper cells - % of CD45+: 14.889

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