Mesenteric Lymph Node Immunophenotyping: Images associated with FACS analysis
WT Images
16 weeks old
Naive CD8+ T cells - % of CD8+: 83.98
16 weeks old
Germinal centre B cells - % of CD45+: 0.564
16 weeks old
CD103+ CD11b-low cDC - % of CD45+: 0.367
16 weeks old
Conventional DC (cDC) - % of DC: 84.824
16 weeks old
CD11b-low cDC - % of cDC: 78.143
16 weeks old
Effector NK cells - % of CD45+: 0.188
16 weeks old
Total ab T cells - % of total T cells: 97.723
16 weeks old
Eosinophils - % of CD45+: 0.082
16 weeks old
Resting CD8+ T cells - % of CD45+: 3.191
16 weeks old
Total gd T cells - % of total T cells: 2.277
16 weeks old
Late germinal centre B cells - % of CD45+: 0.215
16 weeks old
Total NKT cells - % of CD45+: 0.358
16 weeks old
Effector CD4+ NKT cells - % of CD45+: 0.108
16 weeks old
CD4+ T helper cells - % of CD4+: 86.767
16 weeks old
Germinal centre B cells - % of B2 cells: 1.446
16 weeks old
Plasmacytoid DC - % of DC: 15.176
16 weeks old
CD11b-high cDC - % of cDC: 21.518
16 weeks old
Total gd T cells - % of CD45+: 1.301
16 weeks old
Total B2 cells - % of CD45+: 39.036
16 weeks old
Conventional DC (cDC) - % of CD45+: 0.692
16 weeks old
Early germinal centre B cells - % of CD45+: 0.35
16 weeks old
Total CD4+ T cells - % of CD45+: 31.099
16 weeks old
KLRG1+ CD4- NKT cells - % of CD45+: 0.002
16 weeks old
Granulocytes - % of CD45+: 0.015
16 weeks old
KLRG1+ Treg cells - % of CD45+: 0.114
16 weeks old
Resting NK cells - % of NK cells: 60.353
16 weeks old
KLRG1+ CD4+ T helper cells - % of CD45+: 0.073
16 weeks old
Total ab T cells - % of CD45+: 55.836
16 weeks old
Total NK cells - % of CD45+: 0.472
16 weeks old
Resting CD8+ T cells - % of CD8+: 13.092
16 weeks old
Resting gd T cells - % of CD45+: 1.083
16 weeks old
Naive CD8+ T cells - % of CD45+: 20.47
16 weeks old
KLRG1+ CD8+ T cells - % of CD8+: 0.161
16 weeks old
Resting NK cells - % of CD45+: 0.285
16 weeks old
Total DC - % of CD45+: 0.816
16 weeks old
CD5+ gd T cells - % of CD45+: 0.832
16 weeks old
Follicular B cells - % of CD45+: 37.52
16 weeks old
CD11b-low cDC - % of CD45+: 0.541
16 weeks old
KLRG1+ Treg cells - % of Treg: 2.76
16 weeks old
Plasma cells - % of B2 cells: 0.061
16 weeks old
CD4+ T helper cells - % of CD45+: 26.983
16 weeks old
KLRG1+ NK cells - % of NK cells: 13.428
16 weeks old
Total B cells - % of CD45+: 39.969
16 weeks old
KLRG1+ CD4+ T helper cells - % of CD4+ T helper cells: 0.269
16 weeks old
Resting Treg cells - % of Treg: 40.51
16 weeks old
Plasmacytoid DC - % of CD45+: 0.124
16 weeks old
Effector CD4+ T helper cells - % of CD45+: 4.258
16 weeks old
Effector gd T cells - % of gd T cells: 13.682
16 weeks old
Monocytes - % of CD45+: 0.09
16 weeks old
KLRG1+ CD8+ T cells - % of CD45+: 0.039
Mutant Images
15 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Follicular B cells - % of B2 cells: 62.127
15 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Early germinal centre B cells - % of CD45+: 11.792
15 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Germinal centre B cells - % of B2 cells: 29.071
15 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Late germinal centre B cells - % of CD45+: 0.001
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Early germinal centre B cells - % of CD45+: 4.962
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Germinal centre B cells - % of B2 cells: 22.945
15 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Germinal centre B cells - % of CD45+: 11.794
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Germinal centre B cells - % of B2 cells: 10.166
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Early germinal centre B cells - % of CD45+: 3.92
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Early germinal centre B cells - % of CD45+: 10.588
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Follicular B cells - % of B2 cells: 84.799
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Germinal centre B cells - % of CD45+: 4.962
15 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Follicular B cells - % of B2 cells: 53.596
15 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Late germinal centre B cells - % of CD45+: 0.002
15 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Early germinal centre B cells - % of CD45+: 8.675
15 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Germinal centre B cells - % of B2 cells: 34.723
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Late germinal centre B cells - % of CD45+: 0.0
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Germinal centre B cells - % of B2 cells: 10.157
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Late germinal centre B cells - % of CD45+: 0.0
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Germinal centre B cells - % of CD45+: 10.588
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Follicular B cells - % of B2 cells: 73.904
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Germinal centre B cells - % of CD45+: 3.92
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Follicular B cells - % of B2 cells: 85.985
15 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Germinal centre B cells - % of CD45+: 8.678
16 weeks old
Aicdatm1b(EUCOMM)Hmgu homozygote
Late germinal centre B cells - % of CD45+: 0.0
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