The glucose tolerance test measures the clearance of an intraperitoneally injected glucose load from the body. It is used to detect disturbances in glucose metabolism that can be linked to human conditions such as diabetes or metabolic syndrome. Animals are fasted for approximately 16 hours, fasted blood glucose levels are determined before a solution of glucose is administered by intra-peritoneal (IP) injection. Subsequently, the blood glucose level is measured at different time points during the following 2 hours.
Ontological description: MP:0005559 - increased circulating glucose level, MP:0005560 - decreased circulating glucose level, MP:0005293 - impaired glucose tolerance, MP:0005292 - improved glucose tolerance, MP:0005291 abnormal glucose tolerance, MP:0000188 - abnormal circulating glucose level.
Minimum number of mutant animals: 7 mice for each sex.
Age of animal: 56 weeks.
Sexual dimorphism: yes.
The information about the date of the experiment, that is the date when the measurement is performed, is an important parameter which is to be submitted in the Experiment xml file (dateOfExperiment="2013-02-28").
Perform a QC check of the glucose meter routinely as outlined in the manual.
Metadata and examples
Metadata |
Example |
Equipment ID |
ID of the machine used when more than 1 is used having same model and manufacturer. E.g. machine 1, machine 2, machine Minnie, machine Mickey Mouse, etc. |
Equipment manufacturer |
Manufacturer of the equipment. E.g. Roche Diagnostics Ltd. |
Equipment model |
Model of the equipment. E.g. Accu-Chek Aviva. |
Mouse restrained |
If the mouse is restrained in order to score its tail for blood analysis. E.g. Yes/No. |
Type of strip |
The strip used to analyse the blood can be for whole blood or for plasma. E.g. Plasma/Whole blood/Plasma, no maltose interference. |
Experimenter ID |
An ID of any format to be used coherently both inside the same procedure and for all procedures. E.g. Harw_001, or 1/2/3. |
Date equipment last calibrated |
Most recent date in which the equipment (or any part of) used in the procedure was subject to a calibration event. |
Version | Type | Req. Upload | Req. Analysis | Annotation | Increment | Option | Ontology Options | Derived | Unit | Data Type | |
---|---|---|---|---|---|---|---|---|---|---|---|
Body Weight TCPLA_IPG_001_001 | 1.0 | simpleParameter | ![]() |
g | FLOAT | ||||||
Blood glucose concentration TCPLA_IPG_002_001 | 1.0 | seriesParameter | ![]() |
![]() |
0153060120 | mg/dl | FLOAT | ||||
Fasted blood glucose concentration TCPLA_IPG_010_001 | 1.1 | simpleParameter | ![]() |
TCPLA_IPG_002_001 0 increment_value | mg/dl | FLOAT | |||||
Initial response to glucose challenge TCPLA_IPG_011_001 | 1.1 | simpleParameter | ![]() |
TCPLA_IPG_002_001 15 increment_value TCPLA_IPG_002_001 0 increment_value - | mg/dl | FLOAT | |||||
Area under glucose response curve TCPLA_IPG_012_001 | 1.1 | simpleParameter | ![]() |
TCPLA_IPG_002_001 area_under_curve | (minutes*mg)/dl | FLOAT |
Version | Type | Req. Upload | Req. Analysis | Annotation | Increment | Option | Ontology Options | Derived | Unit | Data Type | |
---|---|---|---|---|---|---|---|---|---|---|---|
Equipment ID TCPLA_IPG_003_001 | 1.0 | procedureMetadata | ![]() |
TEXT | |||||||
Equipment manufacturer TCPLA_IPG_004_001 | 1.0 | procedureMetadata | ![]() |
![]() |
AbbottRoche DiagnosticLifescanARKRAY factory.IncAnaloxRoche Diabetes Care | TEXT | |||||
Equipment model TCPLA_IPG_005_001 | 1.0 | procedureMetadata | ![]() |
![]() |
32004-02Accu-Chek AvivaAccu-Chek PerformaOne Touch Ultra 2Super Glucocard II GT-1640GLUCOCARD G+meter GT-1820Alphatrak 2GM9Accu-Chek Aviva ConnectAccu-Chek Guide | TEXT | |||||
Mouse restrained TCPLA_IPG_006_001 | 1.0 | procedureMetadata | ![]() |
yesNo | TEXT | ||||||
Type of strip TCPLA_IPG_007_001 | 1.0 | procedureMetadata | ![]() |
![]() |
Whole bloodPlasmaPlasma, no maltose interference | TEXT | |||||
Experimenter ID TCPLA_IPG_008_001 | 1.0 | procedureMetadata | ![]() |
TEXT | |||||||
Date equipment last calibrated TCPLA_IPG_009_001 | 1.0 | procedureMetadata | DATE | ||||||||
Upper limit of the metre TCPLA_IPG_013_001 | 1.0 | procedureMetadata | mg/dl | FLOAT |