Gross Pathology and Tissue Collection IMPC_PAT_002

Purpose

Experimental Design

Equipment

Procedure

Notes

Parameters

Metadata

Version Type Req. Upload Req. Analysis Annotation Increment Option Ontology Options Derived Unit Data Type
Experimenter ID IMPC_PAT_052_002 2.0 procedureMetadata Tick                                         TEXT
Method of sacrifice IMPC_PAT_054_002 2.0 procedureMetadata Tick                         Isoflurane overdoseIsoflurane overdose and exsanguinationCervical dislocationNoneKetamine (110mg/kg)/Xylazine (11mg/kg)/ Antisedan (1mg/kg)Ketamine (110mg/kg)/Xylazine (11mg/kg)Ketamine(100mg/kg)/ Xylazine (10mg/kg )/ Antisedan (1mg/kg)Ketamine (100mg/kg)/Xylazine (10mg/kg)AvertinCarbon dioxideExsanguinationPentobarb (0.1ml)Pentobarb (0.05ml)Pentobarb (0.15ml)Pentobarb (0.2ml)         TEXT
Method of Blood Collection IMPC_PAT_055_002 2.0 procedureMetadata                                 Cardiac puncture1ml EDTA tubes, Braun-Melsungen, GERRetro-orbital punctureNot bled         TEXT
Equipment ID IMPC_PAT_056_002 2.0 procedureMetadata Tick                                         TEXT
Fixative IMPC_PAT_058_002 2.0 procedureMetadata Tick                         10% buffered formalin phosphate4% NBF-24hrsNeutral buffered formalin4% PFA/PBS10% neutral buffer formalin, RT, O/NFormolBouin10% buffered formalin         TEXT
Date equipment last calibrated IMPC_PAT_059_002 2.0 procedureMetadata                                                 DATE
Equipment manufacturer IMPC_PAT_060_002 2.0 procedureMetadata Tick                         OhausLeicaKeyence Co., LtdOlympusNikonMettler Toledo         TEXT
Equipment model IMPC_PAT_061_002 2.0 procedureMetadata Tick                         AV2101ASP200SVHX-100AV213CSC100Z6 ApoEM6511 x AW 1101 x 4500SZ61C-5060MS104SAV313M165CCoolpix P520         TEXT
Date of sacrifice IMPC_PAT_030_002 2.0 procedureMetadata Tick                                         DATETIME
ID for sample banking protocol IMPC_PAT_031_002 2.0 procedureMetadata Tick                                         TEXT
Balance manufacturer IMPC_PAT_033_001 1.0 procedureMetadata                                 A&DSartoriusOhaus         TEXT
Balance model IMPC_PAT_034_001 1.0 procedureMetadata                                 HR-100ASecuraAdventurer AX223/EAV313AV213C         TEXT
Camera resolution IMPC_PAT_035_001 1.0 procedureMetadata                                                 TEXT
Eye fixative IMPC_PAT_036_001 1.0 procedureMetadata                                                 TEXT