Phenotyping Protocols for Pipeline: IMPC Pipeline IMPC_001

Unrestricted	Mandatory Body Weight IMPC_BWT_001 The body weight test measures the weight of the mouse in a time series, allowing monitoring of its evolutio... continue reading Protocol... View Parameters View Ontology Annotations View Change History Mandatory Fertility of Homozygous Knock-out Mice IMPC_FER_001 To assess the fertility of homozygous knockout mice. ... continue reading Protocol... View Parameters View Ontology Annotations View Change History
Week 9	Open Field IMPC_OFD_001 The Open Field test is used to assess anxiety and exploratory behaviors. It is based on the natural tendenc... continue reading Protocol... View Parameters View Ontology Annotations View Change History Mandatory Combined SHIRPA and Dysmorphology IMPC_CSD_001 SHIRPA and dysmorphology were originally always separate assessments.  However they have recently been... continue reading Protocol... View Parameters View Ontology Annotations View Change History Mandatory Grip Strength IMPC_GRS_001 The grip strength test is used to measure the neuromuscular function as maximal muscle strength of forelimbs... continue reading Protocol... View Parameters View Ontology Annotations View Change History
Week 10	Mandatory Acoustic Startle and Pre-pulse Inhibition (PPI) IMPC_ACS_001 The acoustic startle response is characterized by an exaggerated flinching response to an unexpected strong ... continue reading Protocol... View Parameters View Ontology Annotations View Change History
Week 11	Mandatory Indirect Calorimetry IMPC_CAL_001 Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditu... continue reading Protocol... View Parameters View Ontology Annotations View Change History
Week 12	Electrocardiogram (ECG) IMPC_ECG_001 To provide a high throughput method to obtain Electrocardiograms in a conscious mouse. ... continue reading Protocol... View Parameters View Ontology Annotations View Change History Echo IMPC_ECH_001 To assess the functionality of the heart in order to determine the presence of a mutant phenotype. ... continue reading Protocol... View Parameters View Ontology Annotations View Change History
Week 13	Mandatory Intraperitoneal glucose tolerance test (IPGTT) IMPC_IPG_001 The glucose tolerance test measures the clearance of an intraperitoneally injected glucose load from the bod... continue reading Protocol... View Parameters View Ontology Annotations View Change History Challenge Whole Body Plethysmography IMPC_CHL_001 The purpose of this procedure is to record the respiratory function of mice, when submitted to methacholine ... continue reading Protocol... View Parameters View Ontology Annotations View Change History
Week 14	Mandatory Auditory Brain Stem Response IMPC_ABR_001 Auditory brainstem response test determines hearing sensitivity and other physiological parameters using evo... continue reading Protocol... View Parameters View Ontology Annotations View Change History Mandatory Body Composition (DEXA lean/fat) IMPC_DXA_001 Measure bone mineral content and density as well as body composition in mice using the DEXA (Dual Energy X-... continue reading Protocol... View Parameters View Ontology Annotations View Change History Mandatory X-ray IMPC_XRY_001 Construct and analyse digital X-ray images in immobilised mice using a Faxitron X-Ray system or NTB digital... continue reading Protocol... View Parameters View Ontology Annotations View Change History Pain Test IMPC_PAI_001Hot Plate... continue reading Protocol... View Parameters View Ontology Annotations View Change History
Week 15	Mandatory Eye Morphology IMPC_EYE_001 To detect abnormalities in eye morphology. ... continue reading Protocol... View Parameters View Ontology Annotations View Change History
Week 16	Mandatory Hematology IMPC_HEM_001 Hematological assessment of blood determines blood cell counts (white blood cells, red blood cells, hemoglob... continue reading Protocol... View Parameters View Ontology Annotations View Change History Mandatory Clinical Blood Chemistry IMPC_CBC_001 Clinical chemistry determines biochemical parameters in plasma including enzymatic activity, specific substr... continue reading Protocol... View Parameters View Ontology Annotations View Change History Insulin Blood Level IMPC_INS_001 The insulin concentration in the blood is an important indicator of diabetes. Ontological description: ... continue reading Protocol... View Parameters View Ontology Annotations View Change History Mandatory FACS Analysis IMPC_FAC_001No description available yet View Parameters View Ontology Annotations View Change History Mandatory Gross Pathology and Tissue Collection IMPC_PAT_001 To perform a complete necropsy to detect and record abnormal external findings and macroscopic alterations ... continue reading Protocol... View Parameters View Ontology Annotations View Change History Mandatory Heart Weight IMPC_HWT_001 To evaluate cardiac size using heart weight and body weight. ... continue reading Protocol... View Parameters View Ontology Annotations View Change History Tissue Embedding and Block Banking IMPC_BLK_001 Collect and fix a standard list of tissues from the complete necropsy (see IMPC Gross Pathology & Tissu... continue reading Protocol... View Parameters View Ontology Annotations View Change History Histopathology IMPC_HIS_001No description available yet View Parameters View Ontology Annotations View Change History Adult LacZ IMPC_ALZ_001 To stain adult mutant  and control tissues for bacterial beta-galactosidase (LacZ) activity in order t... continue reading Protocol... View Parameters View Ontology Annotations View Change History

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