Eye Morphology IMPC_EYE_002

Purpose

To detect abnormalities in eye morphology.

Experimental Design

  • Minimum number of animals :  7M + 7F
  • Age at test: Ideal age = 15 weeks ±3 days.  Minimal age = 14 weeks
  • Sex: We would not expect the results of this test to show sexual dimorphism

Procedure

  1. Examine the anterior of both eyes (e.g. with slit lamp) and record any abnormalities
  2. Test the iris/pupil light response
  3. Image abnormal eyes as a minimum or all eyes if capacity permits
  4. Dilate both eyes
  5. Examine the anterior and posterior of both dilated eyes (e.g. with slit lamp and ophthalmoscope) and record any abnormalities
  6. Image abnormal eyes as a minimum or all eyes if capacity permits

OCT:

  1. Turn on the OCT and start the database
  2. Anaesthetize mouse
  3. Prepare mouse eyes with drops and place contact lens (focal length 10 mm) on the right eye
  4. Enter mouse data in the “Create new patient file” area and switch to the “Acquisition” window
  5. Move the OCT camera to the right position and activate measurement modus
  6. Place mouse collaterally to the OCT camera on the right side of a platform that is fixed in front of the OCT lens
  7. Search the contact lens in the live picture of the fundus image field and place the pupil of the mouse eye in the centre of the window
  8. Move the OCT camera such that OCT lens and contact lens touch each other
  9. Focus the fundus picture by slightly moving up/down or forward/backward
  10. Save fundus images
  11. Set the "Ref.Arm“ ruler such that the section of the retina is placed in the centre of the blue rectangle
  12. Set the mode of measurement on "vertical, horizontal line“
  13. Move the blue horizontal line in the fundus image field to the optic nerve level
  14. Save images of retinal sections
  15. Move the OCT camera to the left position
  16. Repeat measurement procedure for the left eye

Scheimpflug Imaging:

  1. Turn on the Pentacam and start the patient data management
  2. Apply one drop 0.5% Atropine to each mouse eye for pupil dilation
  3. Enter mouse data in the “Patient” group box and switch to the Scan menu
  4. Activate the “1 Picture” modus in the “Image Options” area
  5. Move Pentacam to the right position
  6. Hold the mouse on a platform such that the vertical LED 475 nm light slit is orientated in the center of the right eye ball
  7. Guarantee optimal focus by using the fine adjustment software tool in the adjustment window
  8. Start imaging manually by pressing the “Start Scan” button
  9. Scheimpflug images are saved automatically
  10. Move Pentacam to the left position
  11. Repeat measurement procedure for the left eye

Notes

  • As a minimum, all abnormalities should be imaged.
    • Where capacity permits, all mice can be imaged
  • Majority of parameters can be analysed using the standard approach for assessing categorical data. To increase power for analysis purposes, where an abnormality is detected in the left, right or both eyes, the data may be combined to generate one "abnormal" category.

Data QC

Image QC is typically performed during data collection to ensure high quality images are captured whilst eyes are dilated etc.

Parameters & Metadata

Name Version Type Req. Upload Req. Analysis Annotation Increment Option Derived Unit Data Type
Name Version Type Req. Upload Req. Analysis Annotation Increment Option Derived Unit Data Type