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Set up timed mating with heterozygous animals. Dissect at a consistent time and collect >=2 homozygote embryos. Coordination with viability screen is at the centres discretion.
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Score embryos as live or dead if possible.
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Assess embryos according to Gross Morphology parameters.
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Generate gross images of embryos (optional) with scored defects and control embryos.
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Collect tissue for genotyping
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Process embryos for Histopathology, or other imaging (OPTIONAL - depending on center pipeline)
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Scores will be shown per embryo and split by zygosity.
If capturing images please attempt to capture left, right, front, and back views of the embryo but if this is not possible left, right is sufficient. Feel free to take higher magnification views to show morphologies of interest.