Buffy coat peripheral blood leukocyte immunophenotyping MGP_BCI_001

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Purpose

Buffy coat peripheral blood leukocyte immunophenotyping

Procedure

Mice were fed on Mouse Breeder Diet (5021,Labdiet) from weaning

Analysis is performed on the buffy coat obtained from heparinised blood after red blood cell lysis for the following parameters: Percentages of total T cells (CD3+), CD4+ and CD8+ T cells, NKT cells (CD3+ CD161+), NK cells (CD3-CD161+), B cells (CD19+), Granulocytes (CD11b+ Gr1+) and Monocytes (CD11b+ Gr1dim/neg CD161-) are presented relative to the total viable CD45+ population.

Percentages of CD44hi CD62Llo CD4+ T cells and CD25+ regulatory T cells are presented relative to the total CD4+ T cell population.

Percentages of effector of CD44hi CD62Llo CD8+ T cells and mature IgD+ B cells are presented relative to the total CD8+ T cell and B cell populations respectively.

All samples are acquired on a BD LSR II with dead cells excluded with the use of a viability indicator.

All analysis is performed using FlowJo software.

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